a Record control number 000326660700055

a ISSN 0141-8130

a Original cataloging agency NEU GrandLibrary

a Language code of text/sound track or separate title eng

a Classification number QU4

b Item number .P44 2013

a Personal name Adalı, Terin,

h Medium Electronic Document

a Title PEG-calf thymus DNA interactions: Conformational, morphological and spectroscopic thermal studies

c Statement of responsibility, etc Terin Adalı, Ali Bentaleb, Nagib Elmarzugi, Amal M. Hamza.

b Name of publisher, distributor, etc Elsevıer Scıence Bv,

c Date of publication, distribution, etc 2013.

a Summary, etc The main aim of this study is to provide understanding for the interaction modes and the binding affinity based on the study of PEG 400 that binds to ctDNA. The effects of the PEG-400-to-ctDNA ratio, pH, incubation time and thermal stability of ctDNA on PEG-ctDNA biocomplex formation were studied. UV-vis-NIR absorption analysis indicated that PEG forms a complex with ctDNA via a mechanism other than intercalation. The results of thermal denaturation studies showed that the PEG-ctDNA biocomplex helix was stabilised, with a resulting increase in the PEG-ctDNA melting temperature. FTIR analysis indicated that the PEG binds to ctDNA through weak to moderately strong hydrophilic and hydrophobic interactions with the base pairs of ctDNA. TEM micrographs showed that the addition of PEG to ctDNA caused ctDNA to condense with PEG molecules into an irregular aggregate structure. These results demonstrate that the PEG-ctDNA biocomplex has potential applications in biomedical sciences. (C) 2013 Elsevier B.V. All rights reserved.

a Topical term or geographic name as entry element Near East University Article

a Topical term or geographic name as entry element Yakın Doğu Üniversitesi Makale

a Topical term or geographic name as entry element Biochemistry

a Topical term or geographic name as entry element ctDNA;

a Topical term or geographic name as entry element Gene therapy.

a Topical term or geographic name as entry element Conformational studies

a Topical term or geographic name as entry element Tissue engineering

a Topical term or geographic name as entry element Copolymers

a Topical term or geographic name as entry element Pegylatıon

a Topical term or geographic name as entry element Gene Delıvery;

g Relationship information Oct 2013, Vol.61 p.373-378

t Title Internatıonal Journal Of Bıologıcal Macromolecules

x International Standard Serial Number 01418130

u Uniform Resource Identifier http://library.neu.edu.tr:2048/login?url=http://dx.doi.org/10.1016/j.ijbiomac.2013.07.024